Equation of state and high-pressure phase behaviour of SrCO3

The high-pressure phase transition of strontianite (SrCO3) was investigated at ambient temperature by means of powder and single-crystal X-ray diffraction. The samples were compressed in a diamond anvil cell to a maximum pressure of 49 GPa. Structure refinements confirm the existence of SrCO3 in the low pressure aragonite-type phase Pmcn (62) up to about 26 GPa. Above this pressure, SrCO3 transforms into a high-pressure phase with post-aragonite crystal structure Pmmn (59). Fitting the volume extracted from the compression data to the third-order Birch–Murnaghan equation of state for the low-pressure phase of SrCO3 yieldsK0 = 62.7(6)GPa and K ′ 0 = 3.2(1), and for the high-pressure phase this yields K0 = 103(10)GPa and K ′ 0 = 2.3(6). The unit cell parameters change non-uniformly, with the c axis being 4 times more compressible than the a and b axes. Our results unequivocally show the existence of a Pmmn structure in SrCO3 above 26 GPa and provide important structural parameters for this phase.


Introduction
Carbonates play a key role in the chemistry and dynamics of our planet. They are directly connected to the CO 2 budget of our atmosphere and have a great impact on the deep carbon cycle (Li et al., 2019;McCammon et al., 2020). Moreover, it is believed that more than 90 % of the planet's carbon content is stored in the Earth's deep interior (Javoy, 1997;Dasgupta and Hirschmann, 2010;Kelemen and Manning, 2015). Indirect evidence for the presence of a deep carbon cycle is given by the existence of carbonatite melts causing metasomatism in the upper mantle (Litasov et al., 2013), by CO 2 in peridotitic and eclogitic systems with implications for deep melting of subducting slabs (Ghosh et al., 2009;Litasov, 2011;Thomson et al., 2016), by mantle minerals (e.g. clinopyroxene, olivine, garnet) hosting carbon-bearing inclusions (Korsakov and Hermann, 2006;Shcheka et al., 2006), and the formation of diamonds. Several studies performed under the conditions of the Earth's mantle showed that carbon is incorporated in carbonates, which are stable phases at such conditions in equilibrium with other mineral phases (Isshiki et al., 2004;Merlini et al., 2012a,b;Boulard et al., 2015;Bayarjargal et al., 2018;Santos et al., 2019). Findings of carbonate inclusions in diamonds from the upper mantle and transition zone further substantiate the existence of carbonates in the deep Earth (Sobolev et al., 1998;Wirth et al., 2009;Brenker et al., 2007;Kaminsky et al., 2009).
The most abundant carbonates entering the subduction zone are Ca, Mg, and Fe carbonates. At the pressuretemperature (PT) conditions expected for subduction zones and the Earth's mantle, carbonates undergo pressure-and temperature-induced structural changes: CaCO 3 , for instance, undergoes several phase transitions, some of them be-coming more relevant to Earth's mantle conditions (Martinez et al., 1996;Santillán and Williams, 2004;Ono et al., 2005a;Oganov et al., 2006;Bayarjargal et al., 2018); other studies have shown the stability and phase transition of dolomite (Zucchini et al., 2017;Solomatova and Asimow, 2017;Efthimiopoulos et al., 2017), which is thought to be the main carbonate phase in subducting slabs. Regarding CaCO 3 , numerous studies on the phase behaviour of aragonite-type CaCO 3 at high pressure reported a phase transition from orthorhombic aragonite (space group Pmcn) into monoclinic CaCO 3 -VII at around 30 GPa and into post-aragonite structure Pmmn at around 40 GPa (Gavryushkin et al., 2017;Bayarjargal et al., 2018).
Meanwhile, strontianite (SrCO 3 -I), which is isostructural to aragonite at room pressure and room temperature conditions and very common in natural carbonates, is considered to have similar phase transitions but is at a lower pressure compared to aragonite due to the larger ionic radius of Sr 2+ (1.31 Å) in comparison with Ca 2+ (1.18 Å) (Shannon, 1976). Recent findings on mineral inclusions in transition zone diamonds showed significant amounts of strontium (Brenker et al., 2007;Kaminsky, 2012), which motivated our investigations of SrCO 3 as a possible stable phase in the deep Earth.
Whilst most of the physical properties of SrCO 3 are well known at ambient conditions (Villiers, 1971;Antao and Hassan, 2009;Nguyen-Thanh et al., 2016;Biedermann et al., 2017b), they have rarely been measured by powder X-ray diffraction at high pressure (Ono et al., 2005b;Wang et al., 2015), with some of them being based on density functional theory (DFT) calculations (Biedermann et al., 2017a;Efthimiopoulos et al., 2019). However, up to now, no singlecrystal X-ray diffraction data were measured on SrCO 3 , thus making it difficult to analyse structural changes at the conditions of the Earth's mantle. At ambient pressure, SrCO 3 has an aragonite-type crystal structure with space group Pmcn (62). This crystal structure consists of planar trigonal [CO 3 ] 2− oxyanions parallel to (001). The cations (e.g. Sr 2+ ) are surrounded by six CO 2− 3 ions in a trigonal prismatic arrangement parallel to the c axis (see Fig. 8a), whereas the carbonate groups are octahedrally surrounded by six cations.The higher coordination number of the cation in the aragonite-group minerals correlates with a larger ionic radius (e.g. Sr 2+ , Ba 2+ , Pb 2+ ) compared to the calcite-group minerals, where the cation is only coordinated six-fold by the oxygen atoms.
A few studies on the pressure-induced phase transitions in SrCO 3 have been performed (Lin and Liu, 1997;Arapan and Ahuja, 2010;Wang et al., 2015;Biedermann et al., 2017a;Efthimiopoulos et al., 2019). They partially disagree regarding both the stability field and the structure of the highpressure phases. Lin and Liu (1997) suggested a phase transition of SrCO 3 to post-aragonite between 32 and 35 GPa by Raman spectroscopy and proposed a space group setting of P 2 1 22 for this phase. In comparison, Ono et al. (2005b) observed a post-aragonite phase by powder X-ray diffraction of SrCO 3 already at 14.5 GPa and at 40 GPa for CaCO 3 (Ono et al., 2005a). In a later study, Ono (2007) could show that the post-aragonite modification in BaCO 3 has to be the same post-aragonite structure as CaCO 3 and SrCO 3 and had to be described in space group setting Pmmn. More recently, Wang et al. (2015) reported a possible high-pressureinduced transition at room temperature of SrCO 3 from Pmcn to P 2 1 2 1 2 between 22.2 and 26.9 GPa and of BaCO 3 from Pmcn to Pmmn between 9.8 and 11.2 GPa. A similar pressure range for a transition to the post-aragonite phase in SrCO 3 was proposed by Biedermann et al. (2017a) using experimental and computational Raman spectroscopy. Later on, the boundary of this phase transition was extended to hightemperature conditions using mid-infrared absorbance and Raman spectroscopy in combination with DFT-based calculations (Efthimiopoulos et al., 2019).
The variety of experimental conditions and techniques in the cited studies leads to results that are difficult to compare and sometimes even contradictory. To clarify these controversies we investigated the structure of pure SrCO 3 up to 49 GPa at ambient temperature by powder and single-crystal X-ray diffraction. This method allows us to precisely determine the crystal structure and to finally resolve the disagreement concerning the correct space group symmetry for the post-aragonite phase.

Synthesis of sample material
For powder X-ray diffraction experiments, we used commercial SrCO 3 powder from Sigma Aldrich Chemical Company (99.995 % purity). The single crystals of pure SrCO 3 strontianite were grown in a Walker-type multi-anvil apparatus at 4 GPa and 1273 K for 24 h using the same SrCO 3 powder as a starting material. The same synthesis has been used in previous studies (Biedermann et al., 2017a,b). The chemical composition of the synthesized sample was determined using a JEOL Hyperprobe JXA-8500F with a field emission cathode at the GFZ Potsdam. Analysis was conducted with an acceleration voltage of 15 kV, a 10 nA beam current, and a < 10 µm focused beam size. As reference-standard materials we used dolomite for CaO and strontianite for SrO. The chemical analysis indicated a concentration for Ca 2+ below the detection limit of 130 ppm (see the Supplement for electron microprobe analysis). In addition, the morphology and chemical composition of the single crystals were studied with scanning electron microscopy. Most of the synthesized single crystals of SrCO 3 were twinned, which is very common for aragonite-type carbonates, as they exhibit pseudohexagonal morphologies with a mirror plane (110) as a twin plane (Bragg, 1924).

Powder X-ray diffraction experiments
For high-pressure X-ray diffraction studies, SrCO 3 powder was pressurized to 49 GPa in a membrane-driven Mao-Belltype diamond anvil cell (Mao and Hemley, 1997) equipped with 400 µm culets. Drilled pre-indented rhenium gaskets with a hole diameter of 200 µm served as sample chambers. The pressure-transmitting medium (PTM) neon and a ruby sphere were loaded in addition to the sample. The pressure in the cell was measured with an online HR-2000 spectrometer (Ocean Optics) using the R 1 -line fluorescence band shift of ruby described by Mao et al. (1986) directly before and after each measurement, where the average is used as the experimental pressure. In addition, the equation of state of neon was used for additional pressure calibration (Hemley et al., 1989). The powder and single-crystal XRD experiments were carried out at beamline P02.2 at PETRA III, DESY (Hamburg) (Liermann et al., 2015). The X-ray wavelength was λ = 0.2906 Å, and the beam size was 2 × 2 µm 2 at full width at half maximum (FWHM). The calibration of the wavelength and the sample-to-detector distance was performed using standard CeO 2 powder. For data collection, we used a fast flat panel detector XRD1621 from Perkin Elmer (2048 pixels × 2048 pixels with 200 × 200 µm 2 pixel size). The two-dimensional X-ray images were integrated using the Fit2D programme (Hammersley, 2016). Refinements of the powder X-ray diffraction data were performed using the GSAS and EXPGUI software packages (Larson and Von Dreele, 2004).

Single-crystal X-ray diffraction experiments
In the case of high-pressure single crystal X-ray diffraction experiments, a small crystal of SrCO 3 (25-30 µm in diameter and 10 µm thickness), a piece of tungsten (∼ 10 µm in diameter) and a ruby sphere (∼ 10 µm in diameter) were placed into a 150 µm wide cylindrical chamber drilled in a pre-indented rhenium gasket. We used the same type of membrane-driven diamond anvil cells as in the case of powder samples but using diamonds with a smaller culet size of 300 µm. Again, neon was loaded into the cell as a pressure-transmitting medium. The single crystal was pressurized up to pressures of 26.3 GPa. A picture of the single crystal loaded into the cell and compressed to 25.9 GPa is shown in Fig. 1.
The intensities of the reflections for the SrCO 3 single crystal were integrated in steps of 0.5 • over the entire opening angle of the cell of 54 • . At each pressure point, additional X-ray diffraction wide images (from −27 to +27 • in the rotation axis) were collected to confirm the data quality. The instrument model of the experimental geometry (sample-to-detector distance, the detector's origin, offsets of the goniometer angles, and rotation of the X-ray beam and the detector around the in-  (2) Å; c = 5.18320(10) Å). The processing of XRD data (the unit cell determination and integration of the reflection intensities) was performed using CrysAlis PRO software (Rigaku Oxford Diffraction, 2019).
Indexing of the unit cell was carried out on about 30 reflections manually selected in the reciprocal space viewer (Ewald explorer implemented in CrysAlis PRO software). The reflections were selected in order to determine a 3D lattice in the reciprocal space. The identified unit cell parameters were then refined on the whole set of reflections at the end of the integration. . Dots correspond to the measured spectra and the solid red lines represent the best refinements. The difference curves between the measured and the refined patterns are depicted as well (blue curves). Vertical ticks mark the Bragg peak positions. Table 2. Pressure dependence of lattice parameters of SrCO 3 at room temperature derived from powder X-ray diffraction.
SrCO 3 -I in Pmcn 0.0001 5.126(1) 8.472(2) 6.061(1) 263.21(15) 3.6(1) 5.057 (1) The transformation corresponds to two oriented grains rotated by about 118 • around the common c axis. Since the degree of overlap was small (less than 3 % of all reflections), no twin integration was applied for the SrCO 3 -I phase data, and only the most intense grain (e.g. larger volume) was used for extraction of the reflection intensities. Overlapping 00l reflections were missing in the data set due to the orientation of the crystal in the diamond anvil cell (DAC) and its restricted opening angle. However, after the phase transition above 26 GPa we observed three twin domains related to a three-fold rotation along the a axis in SrCO 3 -II. Due to a high degree of overlap between reflections (about 10 %), we applied simultaneous twin integration; however, further structure solution and refinement was performed using the data collected from the most intense twin component. Empirical absorption correction was applied using spherical harmonics, implemented in the SCALE3 ABSPACK scaling algorithm, which is included in the CrysAlis PRO software. The crystal structures of aragonite-type SrCO 3 -I and post-aragonite SrCO 3 -II were determined by the dual space method using SHELXT (Sheldrick, 2015) software. After the structure solution most of the atoms were found and the remaining were located from a series of difference Fourier maps. The crystal structures were refined against F 2 on all data by full-matrix least squares with the SHELXL (Sheldrick, 2015) software. The amount of the collected data allowed us to refine the structures in anisotropic approximation. Nevertheless, there is a pronounced elongation of anisotropic displacement parameters in SrCO 3 -II along the [100] direction, due to threefold twinning about the a axis. Details of crystal structure refinements of SrCO 3 -I and SrCO 3 -II are given in Table 3. The X-ray crystallographic coordinates have been deposited at the Inorganic Crystal Structure Database (ICSD) under deposition no. CSD1944794. This data can be obtained from CCDC's and FIZ Karlsruhe's free service for viewing and retrieving structures (http://www.ccdc.cam.ac.uk/structures/, last access: 28 October 2020).
3 Results and discussion

Phase transition of SrCO 3 during compression
Powder XRD patterns in the 2θ range 3-22 • were collected upon compression up to 49(2) GPa and are presented in Fig. 2. No other peaks except those of the sample and the pressure transmitting medium neon (Ne) were observed. Peaks corresponding to the sample at ambient conditions were well indexed to the low-pressure aragonite structure Pmcn with the lattice parameters a = 5.126(1) Å, b = 8.472(2) Å, and c = 6.061(1) Å and are in agreement with the literature (Wang et al., 2015;Villiers, 1971; Arapan and Ahuja, 2010; Antao and Hassan, 2009). As shown in Fig. 2, reflections of SrCO 3 shift to higher angles with increasing pressure and no structural transformation occurred until 25.9(3) GPa. A Rietveld refinement for the low-pressure aragonite-type SrCO 3 -I phase up to a pressure of 15.1(2) GPa was used to calculate the lattice and structural parameters, including interatomic distances. Above 15 GPa, the broadening of the peaks does not allow reliable refinement of the structure; hence the method of Le Bail fitting was applied in order to derive lattice parameters from powder-XRD measurements between 15 and 49 GPa. Some reflections of SrCO 3 -I disappear, and new peaks appear at 28.5(3) GPa that could not be indexed using the metric of SrCO 3 -I described in space group Pmcn. These new peaks increase in intensity at higher pressures and indicate that SrCO 3 has transformed into a post-aragonite phase. Our observations are in good agreement with powder X-ray diffraction studies from Wang et al. (2015) where a phase transition in SrCO 3 was observed between 22.2 and 26.9 GPa. In contrast, Ono et al. (2005b) proposed the presence of a post-aragonite phase in SrCO 3 already at 14.5 GPa and at high temperature, which was also indexed with the metric of SrCO 3 -II described in space group Pmmn. For CaCO 3 , a phase transformation into post-aragonite was observed at 40 GPa (Ono et al., 2005a) and for BaCO 3 at 10 GPa (Ono, 2007;Townsend et al., 2013). The pressure for a phase transition in SrCO 3 determined here lies in between these values and complies with the pressure-homologue rule according to which isostructural compounds often exhibit similar phase transitions but at lower pressures with increasing ionic radius (Ringwood, 1975).
Single-crystal X-ray diffraction experiments were performed from 0 to 26.3 GPa. In agreement with our results from powder X-ray diffraction, reflections of the single crystal can be unambiguously indexed with the same aragonitetype orthorhombic cell Pmcn between 0 and about 21 GPa. Results taken at 26.3 GPa clearly indicate that SrCO 3 has fully transformed into post-aragonite phase with space group Pmmn, which is the stable phase of SrCO 3 at this higher pressure. The XRD pattern derived from a wide scan of the single crystal at this pressure is shown in Fig. 3. Additional peaks are indexed for rhenium, tungsten and the pressure transmitting medium neon. Fitted d spacings from this study at 26.3 GPa are given in Table 1 and are in good agreement with previous results from Ono et al. (2005b) at 14.5 GPa.
A comparison between the low-pressure phase and the post-aragonite phase is shown in Fig. 4. One can see that the a, b, and c axes in SrCO 3 -I (Pmcn) correspond to the b, c, and a axes in SrCO 3 -II (Pmmn). The phase transition from SrCO 3 -I (Pmcn) to SrCO 3 -II (Pmmn) is a first-order phase transition (Ono et al., 2005a) and is described by a shift of half of Sr 2+ cations and CO 2−  (7) 218.41 (3) 207.39 (7) 203.48 (7) 198.93 (9) (6) 3.8399 (4) 3.7761 (7) 3.7626 (6) 3.7432(10) Sr1· · ·Sr1 in (001)     tion. At the same time, a shift by half of the translation of all Sr 2+ cations and CO 2− 3 anions accompanied with a small displacement along [001] occurs such that the cations and anions line up in one plane parallel to (001). These shifts result in more dense packing of cations and anions and in the increase of the Sr 2+ coordination number from 9 to 12.

Compressibility of SrCO 3 -I and SrCO 3 -II
The refined unit cell parameters for the low-pressure phase strontianite (SrCO 3 -I) and for the high-pressure phase SrCO 3 -II obtained at different pressures are listed in Tables 2 and 3. The evolution of the lattice parameters with pressure is given in Fig. 5. Upon compression from 0.5 to 26 GPa, the axes of SrCO 3 -I change anisotropically with the highest compressibility found in the direction of the c axis, which is perpendicular to the carbonate groups. This anisotropic Figure 6. Evolution with pressure of the Sr· · ·Sr distances in the (001) plane (distance between two grey Sr spheres) and out of the (001) plane (distance between grey and white Sr spheres) in SrCO 3 -I derived from single-crystal X-ray diffraction data. A schematic drawing of the crystal structure of SrCO 3 -I in the (100) plane is given as an inset. compression scheme is typical for aragonite-type carbonates (Townsend et al., 2013;Zhang et al., 2013) and reflects the incompressibility of the CO 3 groups in the (001) plane compared to the SrO 9 polyhedra. The determined lattice parameters for SrCO 3 -I in this study exhibit quasi-linear pressure dependence with no obvious discontinuities. A linear fit of the given a/a 0 , b/b 0 , and c/c 0 values against pressure yields linear compressibilities of β a , β b , and β c with 1.85(2) × 10 −3 , 2.62(7) × 10 −3 , and 6.4(5) × 10 −3 GPa −1 , respectively, and agrees well with data from Wang et al. (2015). Variations of the Sr· · ·Sr distances in SrCO 3 -I upon compression further substantiate an anisotropic compression behaviour, indicating anomalous contraction along the c axis (Fig. 6). Across phase transition at about 26 GPa, the length of the c axis (=a in SrCO 3 -II) sharply decreases by ∼ 10 %, whereas the b axis (=c in SrCO 3 -II) increases. The lattice parameters of SrCO 3 -II further decrease with pressure, albeit at lower rates, indicating that the structure of SrCO 3 -II is less compressible than SrCO 3 -I. The linear compressibilities of the high-pressure phase are found to be 2.223(7) × 10 −3 GPa −1 for the a axis, 1.29(8) × 10 −3 GPa −1 for the b axis, and 1.05(4) × 10 −3 GPa −1 for the c axis.
The pressure dependence of the molar volume for SrCO 3 -I and SrCO 3 -II is shown in Fig. 7a. A plot of the Eulerian strain against the normalized pressure (f-F plot) (Fig. 7b) indicates that a third-order Birch-Murnaghan equation of state is necessary to fit the pressure-volume (P-V) data of SrCO 3 , which  is expressed as follows (Birch, 1947;Angel et al., 2014): with f E as the Eulerian strain given by and where V 0 , K 0 , and K are the (molar) volume, the isothermal bulk modulus, and its pressure derivative at room pressure, respectively. The programme EosFit7c (Angel et al., 2014) was used to fit the data. For the low-pressure phase strontianite (SrCO 3 -I), the following EoS parameters were calculated: V 0 = 259.8(3) Å 3 , K 0 = 62.7(6) GPa, and K 0 = 3.2(1). Values for K 0 are in good agreement with previous studies on strontianite (Wang et al., 2015;Biedermann et al., 2017b). The fit result for the high-pressure phase SrCO 3 -II yields V 0 = 115(1) Å 3 , K 0 = 103(10) GPa and K 0 = 2.3(6), respectively. As indicated in Table 4, these values are between the values for BaCO 3 -II, with K 0 = 84(4) GPa (Ono, 2007), and for post-aragonite CaCO 3 , with 127(14) GPa (Ono et al., 2005a), thus confirming a dependency on the cation radius.

Crystal structure of post-aragonite SrCO 3 -II
A detailed refinement of the high-pressure phase was carried out on single crystal X-ray diffraction data at 26.3 GPa. We were able to refine the structure in the anisotropic approximation. Fractional atomic coordinates and anisotropic displacement parameters are shown in Table 5. At this pressure, the post-aragonite phase of SrCO 3 crystallizes in an orthorhombic unit cell with space group Pmmn (no. 59, origin choice 2) and with the following unit cell parameters: a = 4.543(2) Å,   (3) 0.009(4) 0.000(2) 0 0 b = 4.939(2) Å, c = 4.232(2) Å, and V = 94.97(8) Å 3 . The atomic arrangement in post-aragonite SrCO 3 -II adopts the structure type of the orthorhombic high-pressure form of RbNO 3 -V (Kalliomäki and Meisalo, 1979). The Sr ion is located on a Wyckoff position 2b, C is on a 2a position, and O1 and O2 atoms occupy 2a and 4e positions, respectively (Table 5). The same crystal structure was already proposed for high-pressure modifications of CaCO 3 (Ono et al., 2005a) and for BaCO 3 (Ono, 2007;Townsend et al., 2013). However, there are also XRD data suggesting a trigonal symmetry for the post-aragonite phase in CaCO 3 (Santillán and Williams, 2004) and in BaCO 3 (Holl et al., 2000). When adapting the trigonal symmetry reported by Holl et al. (2000) some diffraction peaks of the high-pressure phase of SrCO 3 could not be indexed. We conclude that for SrCO 3 only an orthorhombic description of the crystal structure for the postaragonite phase is possible.
The Pmcn to Pmmn transition of SrCO 3 is characterized by an increase of the coordination of Sr 2+ from 9 to 12. The relative change in density across this transition is about 5 %. In the post-aragonite phase the Sr to O interatomic distances vary from 2.418(10) to 2.718(6) Å. Six oxygen atoms with shorter distances (2.418(10), 2.4842(19), and 2.613(10) Å) are located on a plane intersecting Sr, parallel to the bc plane. Three oxygen atoms are located below the plane, and three atoms are located above it. C to O distances in the highpressure phase of SrCO 3 are 1.291(13) and 1.31(2) Å and identical within errors with data from Ono et al. (2005a) for CaCO 3 . the high-pressure phase behaviour of SrCO 3 up to 49 GPa at ambient temperature. We observed a transformation from strontianite (Pmcn) to post-aragonite SrCO 3 -II (Pmmn) at around 26 GPa, which is in agreement with previous studies that used other techniques, e.g. Raman spectroscopy, to detect a phase transition in SrCO 3 (Biedermann et al., 2017a). We present reliable structural information for post-aragonite SrCO 3 -II, including interatomic distances and anisotropic displacement parameters, and finally resolve the discussion about the correct space group setting of the post-aragonite phase in SrCO 3 . Our results further confirm a bulk modulus of 62.7(6) GPa for strontianite (SrCO 3 -I) and provide the first experimental data for the equation of state for the high-pressure phase SrCO 3 -II with V 0 = 115(1) Å 3 , K 0 = 103(10) GPa and K 0 = 2.3(6).
Data availability. The electron microprobe analysis of the sample material and the refinement of the single-crystal X-ray diffraction data on the post-aragonite phase of SrCO 3 are included in the Supplement. Further data will be made available upon request for scientific research purposes.
Author contributions. KA and MW initiated the project. IE and JM processed and contributed powder X-ray diffraction data to this study. NB and EB processed single-crystal X-ray diffraction data and wrote the paper with input from WM and IE. EB refined singlecrystal X-ray diffraction data. KA, GS, WM, KG, AP, and MW participated in X-ray diffraction data acquisition.
Competing interests. The authors declare that they have no conflict of interest.